primary antibodies against dnmt3a  (Santa Cruz Biotechnology)

Journal: European Journal of Medical Research

Article Title: MiRNA-29b accelerates the PDGF in exosomes and stimulates hepatic stellate cells to promote liver fibrosis in biliary atresia

doi: 10.1186/s40001-025-02731-z

Figure Lengend Snippet: Effects of miRNA-29b overexpression and inhibition in exosomes on the expression of DNMT3a, DNMT3b, PDGFA, COL1A1, and α-SMA in LX-2 cells and verification of miRNA-29b target genes by dual luciferase assays. a miRNA-29b-overexpressing exosomes were cocultured with LX-2 cells, and the expression levels of DNMT3a and DNMT3b in the Up group were significantly decreased. b The transcription level of PDGFA in the b Up group was significantly increased. c The expression levels of COL1A1 and α-SMA in the Up group were increased. d Exosomes with low miRNA-29b expression were cocultured with LX-2 cells, and the expression levels of DNMT3a and DNMT3b in the Down group were significantly increased. e The expression level of PDGFA in the Down group was decreased. f The expression of COL1A1 and α-SMA decreased in the Down group. g Double luciferase experiments proved that DNMT3a and DNMT3b were the target genes of miR-29b-3p. Note: Normal: untreated LX-2 cells in the blank control group; control: negative empty exosomes were cocultured with LX-2 cells. Down: exosomes with low miRNA-29b expression were cocultured with LX-2 cells; a–f : LX-2 cells cocultured with exosomes from 3 independent experiments (n = 3); g: Data from 4 biological replicates (n = 4). * indicates p < 0.05 compared with the control group

Article Snippet: The membrane was blocked with 5% skim milk and incubated with primary antibodies against DNMT3a, DNMT3b, PDGFA, COL1A1 and α-SMA (GeneTex, Abcam).

Techniques: Over Expression, Inhibition, Expressing, Luciferase, Control

Journal: European Journal of Medical Research

Article Title: MiRNA-29b accelerates the PDGF in exosomes and stimulates hepatic stellate cells to promote liver fibrosis in biliary atresia

doi: 10.1186/s40001-025-02731-z

Figure Lengend Snippet: Effect of 5-AZAC on LX-2 cells cocultured with miRNA-29b overexpression and inhibition exosomes. a The miRNA-29b overexpression exosome + 5Azac group showed decreased expression of DNMT3a and DNMT3b; b the miRNA-29b overexpression exosome + 5Azac group showed increased expression of PDGFA; c The expression of COL1A1 and α-SMA in the miRNA-29b overexpressed exosome + 5Azac group increased; d The expression levels of DNMT3a and DNMT3b in the miRNA-29b low-expression exosome + 5Azac group were decreased; e the miRNA-29b low expression exosome + 5Azac group showed increased expression of PDGFA; f The expression of COL1A1 and α-SMA increased in the miRNA-29b low-expression exosome + 5Azac group. Note : Normal: untreated LX-2 cells in the blank control group; control: negative empty exosomes were cocultured with LX-2 cells. Up + 5Azac: miRNA-29b-overexpressing exosomes + 5Azac were cocultured with LX-2 cells. Down + 5Azac: Exosomes with low expression of miRNA-29b + 5Azac were cocultured with LX-2 cells; a – f : Data from 3 independent experiments (n = 3). * indicates p < 0.05 compared with the control group

Article Snippet: The membrane was blocked with 5% skim milk and incubated with primary antibodies against DNMT3a, DNMT3b, PDGFA, COL1A1 and α-SMA (GeneTex, Abcam).

Techniques: Over Expression, Inhibition, Expressing, Control

Journal: European Journal of Medical Research

Article Title: MiRNA-29b accelerates the PDGF in exosomes and stimulates hepatic stellate cells to promote liver fibrosis in biliary atresia

doi: 10.1186/s40001-025-02731-z

Figure Lengend Snippet: Detection of the relative expression of transcript level and protein level of each gene in UP group and Up + 5Azac group, Down group and Down + 5Azac group. a–b: At both transcriptional and protein levels, the Up + 5Azac group showed significantly lower DNMT3a/3b enzyme activities, higher PDGFA methylation levels, and increased COL1A1 and α-SMA expression compared to the Up group. c-d: At the transcriptional and protein levels, the Down + 5Azac group showed decreased DNMT3a/3b enzyme activity, increased PDGFA methylation levels, and increased COL1A1 and α-SMA expression compared to the Down group. Note: Up: exosomes overexpressing miRNA-29b were cocultured with LX-2 cells; Up + 5Azac: miRNA-29b -overexpressing exosomes + 5Azac were cocultured with LX-2 cells; Down: exosomes with low miRNA-29b expression were cocultured with LX-2 cells; Down + 5Azac: Exosomes with low expression of miRNA-29b + 5Azac were cocultured with LX-2 cells. a – d : LX-2 cells cocultured with exosomes from 6 independent experiments (n = 6). * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; **** indicates p < 0.0001

Article Snippet: The membrane was blocked with 5% skim milk and incubated with primary antibodies against DNMT3a, DNMT3b, PDGFA, COL1A1 and α-SMA (GeneTex, Abcam).

Techniques: Expressing, Methylation, Activity Assay

Journal: EBioMedicine

Article Title: UVB induces cutaneous squamous cell carcinoma progression by de novo ID4 methylation via methylation regulating enzymes

doi: 10.1016/j.ebiom.2020.102835

Figure Lengend Snippet: Sequences of primers used in qPCR.

Article Snippet: The slides were first blocked with normal goat serum at room temperature for 30 min to minimize nonspecific staining, then incubated overnight with primary antibodies against DNMT1 (1:100, Absin Bioscience Inc., Shanghai, China), DNMT3A (1:50, CST, USA), DNMT3B (1:50, Abcam, UK), TET1 (1:100, Absin Bioscience Inc., Shanghai, China), TET2 (1:100, Absin Bioscience Inc., Shanghai, China), and TET3 (1:100, Abcam, UK).

Techniques: Sequencing

Journal: EBioMedicine

Article Title: UVB induces cutaneous squamous cell carcinoma progression by de novo ID4 methylation via methylation regulating enzymes

doi: 10.1016/j.ebiom.2020.102835

Figure Lengend Snippet: Altered expression of DNMTs and TETs in CSCCs compared with adjacent normal tissues. (a) The relative expression levels of DNMTs and TETs in CSCC and adjacent normal tissues. Data are shown as means ± SD. The statistical significance was assessed by the Student's t -test. (b) The Pearson's correlation between expression level (2 −△Ct ) or methylation level of ID4 and expression levels (2 −△△Ct ) of DNMT1, DNMT3A, DNMT3B, TET1, TET2, and TET3, which were measured by MSP and qPCR in the same patient sets.

Article Snippet: The slides were first blocked with normal goat serum at room temperature for 30 min to minimize nonspecific staining, then incubated overnight with primary antibodies against DNMT1 (1:100, Absin Bioscience Inc., Shanghai, China), DNMT3A (1:50, CST, USA), DNMT3B (1:50, Abcam, UK), TET1 (1:100, Absin Bioscience Inc., Shanghai, China), TET2 (1:100, Absin Bioscience Inc., Shanghai, China), and TET3 (1:100, Abcam, UK).

Techniques: Expressing, Methylation

Journal: Molecular Genetics & Genomic Medicine

Article Title: Inhibitory effect of alpinetin on IL‐6 expression by promoting cytosine methylation in CpG islands in the IL‐6 promoter region

doi: 10.1002/mgg3.993

Figure Lengend Snippet: Effect of alpinetin on protein expression of DNMT3A. Expressions of PPAR and DNMT3A in RAW246.7 cells were determined by Western blot assay after cells were treated with alpinetin (0, 50, 100, 200, 500, 1,000 g/ml). Data were expressed as means ± SD . # p < .05 versus control group, ▼ p < .05 versus 1 mg/ml Alp group

Article Snippet: Next, the membranes were incubated with labeled primary antibodies against PPAR and DNMT3A (all dilution at 1:1,000, Cell Signaling Technology, Danvers, MA, USA) at room temperature for 2 hr.

Techniques: Expressing, Western Blot